| Product Name |
Vesicular Stomatitis Virus (VSV) Pseudotyped with SARS-CoV-2 Spike Glycoprotein, HexaPro-Stabilized Trimer (United Kingdom Variant, N501Y) |
| Description |
Recombinant VSV pseudotyped with SARS-CoV-2 spike glycoprotein, engineered with HexaPro mutations (K986P, V987P, F817P, A892P, A899P, A942P) to stabilize the spike protein in its antigenically optimal pre-fusion conformation. The construct includes the N501Y mutation (B.1.1.7/Alpha lineage) and features a furin cleavage site modification (RRAR→GSAS). Produced in HEK293T cells and purified for high-quality pseudovirus preparations. |
| Key Features |
- Spike Stabilization: HexaPro mutations enhance pre-fusion stability for consistent antigen presentation.
- Variant-Specific Mutation: Contains the N501Y substitution (UK/Alpha variant).
- Furin Site Modification: RRAR→GSAS mutation abolishes furin-mediated cleavage.
- Reporter Gene: Encodes enhanced green fluorescent protein (EGFP) for rapid infectivity quantification.
|
| Applications |
- Neutralizing antibody screening against SARS-CoV-2.
- Evaluation of vaccine-elicited immune responses.
- Viral entry mechanism studies and inhibitor assessment.
|
| Source |
HEK293T cells |
| Reporter |
EGFP (enhanced green fluorescent protein) |
| Titer |
> 1 × 107 RFU/mL (relative fluorescence units) or > 1 × 105 TU/mL (transducing units) |
| Shipping |
Dry ice |
| Storage |
–80°C |
| References |
Hoffmann et al. (2020). Generation of VSV Pseudotypes Using Recombinant ΔG-VSV for Studies on Virus Entry, Identification of Entry Inhibitors, and Immune Responses to Vaccines. Sci Rep 10, 19076. doi:10.1016/j.jviromet.2010.08.006 |