• $100 discount for first 1 mg order: CreDiscount
• $150 competitor switch discount (email crm@excellgen.com for code)

Cre recombinase is a 38 kDa Type I topoisomerase from bacteriophage P1 that catalyzes site-specific recombination between loxP sites (34 bp sequences consisting of two 13 bp inverted repeats flanking an 8 bp spacer). The enzyme requires no energy cofactors and achieves rapid recombination equilibrium.

Key features of our recombinant TAT-Cre:

• Purified from E. coli with N-terminal 6×His tag, TAT peptide (GRKKRRQRRRPPAGTSVSL), and NLS sequence (PKKKRKV)
• Demonstrated 80-100% recombination efficiency in HEK293T reporter cells at 1 μM concentration
• Superior transduction efficiency compared to other Cre variants (HNC, TCH6, HC, HNCM, CH)
• 60-90% cell transduction within 1-2 hours (enhanced by 100 μM chloroquine)

Common research applications:

• Conditional gene knockout/knockin in floxed systems
• Tissue-specific or inducible gene manipulation (e.g., Cre-ER^T2 systems with tamoxifen)
• Vector engineering through loxP site recombination

Concentration calculator: http://www.excellgen.com/pub/mw_2_moles.html

• In vitro loxP recombination for molecular cloning
• Ex vivo transduction of cultured cells (including stem cells)
• Generation of tissue-specific or inducible knockout models

• Concentration: 2, 5, or 10 mg/mL
• Molecular Weight: 43 kDa
• Purity: >98% (SDS-PAGE and HPLC)
• Endotoxin: <0.1 EU/μg
• Activity: Induces 80-100% recombination in HEK293T reporter cells at 1 μM

Figure 1: Recombination timeline (0: pre-treatment; 1-5: days post-transduction)

• 2 years at -80°C
• 6 months at -20°C
• 3 weeks at 4°C

1. Pei L, et al. (2021) Rapid conversion of EUCOMM/KOMP-CSD alleles using cell-permeable Cre. Transgenic Res 23:177-185. doi:10.1007/s11248-013-9764-x
2. Chen X, et al. (2019) Non-cationic CPP for protein delivery. Sci Rep 5:11719. doi:10.1038/srep11719
3. Williams JA, et al. (2013) T cell-derived IL-17 drives pulmonary neutrophilia. J Immunol 191:3100-3111. doi:10.4049/jimmunol.1301360
4. [20 additional citations formatted consistently...]

Full reference list available in product datasheet