| Product Name |
Inorganic Pyrophosphatase (E. coli, Recombinant) |
| Catalog Number |
EG-193 |
| Description |
Recombinant inorganic pyrophosphatase from Escherichia coli, expressed in E. coli and purified to high homogeneity. This enzyme catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate (Pi), a critical reaction for driving biosynthetic processes and molecular biology workflows.
The enzyme is supplied in a buffered solution and is free of detectable exonuclease and endonuclease activities.
|
| Synonyms |
Pyrophosphate phosphohydrolase, PPase, ppa, b4226, JW4185 |
| Tag |
N-terminal 6xHis (if applicable) |
| Expression System |
E. coli |
| Molecular Weight |
~20–22 kDa (monomer); native enzyme is a homohexamer (~120 kDa)[1][7] |
| Purity |
≥95% by SDS-PAGE[5][7] |
| Specific Activity |
≥3500 U/mg (typical)[5][6] |
| Cofactor |
Mg2+ required for activity[1][5][6] |
| Buffer/Formulation |
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 10% glycerol, 50 mM NaCl[5][7] |
| Applications |
- In vitro transcription (IVT) and RNA synthesis reactions
- Removal of inhibitory pyrophosphate in DNA/RNA polymerase reactions
- Biochemical and metabolic studies
- Enzyme-coupled assays
|
| Unit Definition |
One unit liberates 1 µmol of phosphate per minute from inorganic pyrophosphate at 37°C, pH 8.5[5][6] |
| Storage |
-20°C (long term) |
| Shipping |
Dry ice |