| Product Name |
mRNA Cap 2´-O-Methyltransferase (Recombinant) |
| Catalog Number |
EG-185 |
| Description |
Recombinant mRNA Cap 2´-O-Methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to the 2´-O position of the first nucleotide adjacent to the cap structure (Cap-0) at the 5´ end of RNA, converting it to a Cap-1 structure. This modification is critical for mRNA stability, enhanced translation efficiency, and evasion of innate immune responses in eukaryotic cells[1][2][6][7].
|
| Source |
Recombinant E. coli expressing the vaccinia virus gene[2][5][6][7]. |
| Tag |
N/A or 6xHis (varies by supplier) |
| Purification |
Affinity and/or ion exchange chromatography (FPLC) |
| Unit Definition |
One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt capped RNA transcript in 1 hour at 37°C[2][5][6][7].
|
| Concentration |
Typically 50 U/μL[2][5][6][7]. |
| Buffer/Formulation |
20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol[5][6].
|
| Applications |
- In vitro capping of mRNA for research and therapeutic applications
- Generation of Cap-1 mRNA for improved translation and stability
- Preparation of mRNA for transfection, microinjection, or vaccine manufacturing
|
| Quality |
- ≥95% purity by SEC-HPLC
- Negative for DNase, RNase, and protease
- Endotoxin: ≤5 EU/mL
|
| Storage |
-20°C or lower; avoid repeated freeze-thaw cycles[2][5][6]. |
| Shipping |
Shipped on ice or dry ice[2][5]. |