| Product Name |
RNase Inhibitor, Sus scrofa (Pig, Recombinant) |
| Catalog Number |
EG-155 |
| Description |
Recombinant ribonuclease inhibitor (RNH1) from Sus scrofa (pig), expressed in E. coli and purified to high homogeneity. This cytoplasmic protein binds and potently inhibits RNase A-type ribonucleases (RNase A, B, C, and angiogenin) in a 1:1 ratio, protecting RNA from degradation in molecular biology and diagnostic workflows[1][4][5][6].
|
| Synonyms |
RNH1, Ribonuclease inhibitor, RNase inhibitor[1][4] |
| Molecular Weight |
~50 kDa (monomer)[5][6] |
| Expression System |
E. coli |
| Purity |
≥97% by SDS-PAGE[5][6] |
| Unit Definition |
One unit inhibits the activity of 0.375 ng of RNase A by ≥95%[5][6].
|
| Buffer/Formulation |
20 mM HEPES-KOH (pH 7.6), 50 mM KCl, 0.1 mM EDTA, 8 mM DTT, 50% glycerol[6].
|
| Applications |
- Protection of RNA in RT-PCR, RT-qPCR, and cDNA synthesis
- RNA sequencing and single-cell RNA-seq workflows
- In vitro transcription (IVT) and RNA sample preparation
- Any application where RNA integrity is critical
|
| Properties |
- Active at temperatures up to 55°C[6]
- Heat inactivation: 70°C for 20 min[6]
- Does not inhibit RNase T1, S1 nuclease, RNase H, or RNase from Aspergillus[5]
|
| Quality Control |
- No detectable DNase, RNase, or protease contamination[5][6]
- Endotoxin: ≤5 EU/mL
|
| Storage |
-20°C (long term), avoid repeated freeze-thaw cycles[5][6] |
| Shipping |
Dry ice |
|