SARS-Cov-2 Spike Glycoprotein Receptor Binding Domain, RBD (319-541), His tag

Catalog #: EG-302

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Cat #	EG-301
Description	A recombinant form of the spike glycoprotein receptor binding domain (RBD, Arg319-Phe541) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced in HEK293 cells and purified by nickel affinity and ion exchange chromatography. This recombinant protein contains 223 residues of the SARS-CoV-2 spike glycoprotein RBD with a C-terminal hexa-histidine tag.
Mutations	WT referencing Wuhan-Hu-1
Tags	C-terminal hexa-histidine
Purification	FPLC by nickel affinity and ion exchange chromatography
Sequence	RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVL YNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKI ADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDI STEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELL HAPATVCGPKKSTNLVKNKCVNFGSHHHHHH
Mol Wt	25.9 kDa
Source	HEK 293
Purity	90~95% by SDS-PAGE
Applications	Western blot, ELISA and animal vaccination
Storage Buffer	PBS + 50% Glycerol
Storage	-20ºC ~ -80ºC
Shipping	dry ice
References

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