| Product Name |
Tn5 Transposase |
| Size |
100 µg |
| Description |
Tn5 Transposase is a hyperactive, recombinant enzyme derived from the Tn5 transposon of Escherichia coli. It catalyzes the efficient, sequence-independent integration (“tagmentation”) of double-stranded DNA by simultaneously fragmenting and tagging DNA with sequencing adapters. Tn5 is widely used in next-generation sequencing (NGS) library preparation workflows, including ATAC-seq, ChIPmentation, and other applications where rapid and uniform DNA fragmentation and tagging are required.
|
| Applications |
- Next-generation sequencing (NGS) library construction
- ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing)
- ChIPmentation
- Rapid DNA fragmentation and adapter tagging
- Genome mapping and functional genomics studies
|
| Source |
Recombinant protein expressed in E. coli |
| Fusion Tag |
N-terminal 6XHis tag |
| Purification Method |
Affinity chromatography (Ni-NTA) and FPLC |
| Purity |
>95% as determined by SDS-PAGE |
| Storage Buffer |
20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol
|
| Storage |
-20 °C to -80 °C (long-term); avoid repeated freeze-thaw cycles |
| Shipping |
Shipped on ice packs (4 °C) or dry ice |