| Service Name |
AAVExpressTM AAV Production and Purification |
| Description |
AAVExpressTM provides fast, high-quality adeno-associated virus (AAV) production and purification services for research and gene therapy applications. Our platform delivers ultra-pure, high-titer AAV particles using advanced packaging, iodixanol gradient ultracentrifugation, and FPLC-based anion-exchange chromatography. We offer a broad range of serotypes and customization options to meet your experimental needs.
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| Key Features & Advantages |
- Fast turnaround: 2–6 weeks from plasmid to purified AAV
- High titer: up to 1×1013 genomic copies/ml
- Multiple serotypes available: AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV-DJ, AAV-DJ/8
- Custom promoters: hCMV, mCMV, CAG, EF-1α, CMV-intron, CMV5, and more
- Optional self-complementary AAV (scAAV) vector for double-stranded virus production
- Ultra-pure AAV via iodixanol gradient ultracentrifugation and FPLC purification
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| Workflow |
- Cloning of gene of interest (GOI) into cis-plasmid vector (if required)
- Large-scale transfection and AAV packaging
- Purification by iodixanol step gradient ultracentrifugation and FPLC
- Titer determination (genomic copies/ml)
- Optional: gene targeting or in vivo delivery studies
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| Required Materials |
- Endotoxin-free plasmid DNA containing the gene of interest (GOI)
- or sequence of GOI for cloning (additional fee applies)
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| Deliverables |
- Ultra-pure, high-titer AAV viral particles (up to 1×1013 gc/ml)
- Quality control data (titer, purity, serotype confirmation)
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| Applications |
- In vitro and in vivo gene delivery
- Gene therapy research
- Gene targeting and functional studies
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Quotation & Ordering |
- Request a quotation form from customer service: crm@excellgen.com
- Submit the completed form and PO/credit card info by email, fax, or phone
- Ship samples with a hard copy of the completed form to:
Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743
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