| Product Name |
Anti-B7-H6 Monoclonal Antibody, Recombinant |
| Catalog # |
EG-370 |
| Size |
100 µg |
| Key Features |
- Rabbit monoclonal antibody produced in HEK293 cells under serum-free, protein-free conditions
- Purified by Protein A affinity chromatography
- High batch-to-batch consistency and reproducibility
- Long-term supply security
|
| Clone Number |
M18 |
| Immunogen |
Recombinant extracellular domain of human B7-H6 expressed and purified from HEK293 cells |
| Target |
Human B7-H6 (extracellular region) |
| Species Cross-Reactivity |
Human |
| Applications |
Flow Cytometry, Immunoprecipitation (IP), ELISA |
| Recommended Working Concentrations |
- Flow Cytometry / ELISA: 5 µg/mL final concentration
- Immunoprecipitation: 10 µg per 0.3–0.5 mg cell lysate
|
| Background |
B7 homolog 6 (B7-H6) is a recently identified member of the B7 family, expressed on the surface of tumor cells. B7-H6 contributes to tumorigenesis by inhibiting apoptosis, promoting proliferation, and modulating immune responses. B7-H6 is considered a promising biomarker and therapeutic target for cancer immunotherapy, diagnostics, and prognostics.
|
| Quality Control |
Flow cytometry assay demonstrating cell binding intensity. ZR5 cells (1 × 106) were incubated with 5 µg anti-B7-H6 monoclonal antibody and detected with a Cy5-conjugated goat anti-rabbit secondary antibody. Shaded area: isotype control; cyan line: B7-H6 antibody staining.
|
| Concentration |
As indicated on the vial label |
| Formulation |
80% PBS, pH 7.4; 20% glycerol; 5% trehalose; 0.04% sodium azide (preservative) |
| Shipping |
Shipped on blue ice |
| Storage |
-20 to -70 °C (long-term)
4 °C (up to one week)
For frequent use, aliquot to avoid repeated freeze-thaw cycles.
|