| Product Name |
Anti-B7-H3 Monoclonal Antibody, Recombinant |
| Catalog # |
EG-372 |
| Size |
100 µg |
| Key Features |
- Rabbit monoclonal antibody produced in HEK293 cells under serum-free, protein-free conditions
- Purified by Protein A affinity chromatography
- High batch-to-batch consistency and reproducibility
- Long-term supply security
|
| Clone Number |
C10 |
| Immunogen |
Recombinant extracellular domain of B7-H3 expressed and purified from HEK293 cells |
| Target |
Extracellular region of B7-H3 (CD276) |
| Species Cross-Reactivity |
Human |
| Applications |
Flow Cytometry, Immunoprecipitation (IP), ELISA |
| Recommended Working Concentrations |
- Flow Cytometry / ELISA: 5 µg/mL final concentration
- Immunoprecipitation: 10 µg per 0.3–0.5 mg cell lysate
|
| Background |
B7-H3 (CD276) is a member of the B7 family and is overexpressed in many cancer types. As an immune checkpoint protein, B7-H3 inhibits tumor antigen-specific immune responses and contributes to tumor progression by promoting migration, invasion, angiogenesis, chemoresistance, epithelial-to-mesenchymal transition (EMT), and metabolic regulation.
B7-H3 is considered an attractive target for cancer immunotherapy, with several therapeutic agents in development, including enoblituzumab (MGA271), omburtamab, MGD009, MGC018, DS-7300a, and CAR T cells.
Reference: Kontos F, et al. B7-H3: an attractive target for antibody-based immunotherapy. Clinical Cancer Research. 2020, 27:1227–1235.
|
| Quality Control |
Flow cytometry assay demonstrating cell surface expression of B7-H3. KLM-1 cells (1 × 106) were incubated with 5 µg antibody in 1 mL PBS containing 5% BSA. Antibody binding was detected using a Cy5-conjugated goat anti-rabbit secondary antibody. Blue line: isotype control; red line: B7-H3 antibody staining.
|
| Concentration |
As indicated on the vial label |
| Formulation |
80% PBS, pH 7.4; 20% glycerol; 5% trehalose; 0.04% sodium azide (preservative) |
| Shipping |
Shipped on blue ice |
| Storage |
-20 to -70 °C (long-term)
4 °C (up to one week)
For frequent use, aliquot to avoid repeated freeze-thaw cycles.
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