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Anti-FAT1 monoclonal antibody, recombinant

Cat #: EG-371

$250.00

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Product Name Anti-FAT1 Monoclonal Antibody, Recombinant
Catalog # EG-371
Size 100 µg
Key Features
  • Rabbit monoclonal antibody produced in HEK293 cells under serum-free, protein-free conditions
  • Purified by Protein A affinity chromatography
  • High batch-to-batch consistency and reproducibility
  • Long-term supply security
Clone Number M5
Immunogen Recombinant extracellular FAT1 fragment proximal to the transmembrane domain, expressed and purified from HEK293 cells
Target Extracellular region near the transmembrane domain of human FAT1
Species Cross-Reactivity Human
Applications Flow Cytometry, Immunoprecipitation (IP), ELISA
Recommended Working Concentrations
  • Flow Cytometry / ELISA: 5 µg/mL final concentration
  • Immunoprecipitation: 10 µg per 0.3–0.5 mg cell lysate
Background Protocadherin FAT1 is a large single-pass transmembrane protein whose extracellular domain comprises 34 cadherin repeats, 5 EGF-like domains, and a laminin-G-like domain. The intracellular domain contains a nuclear localization signal and regulates gene expression upon activation. FAT1 modulates Hippo, TGF-β, and Wnt/β-catenin signaling pathways in colorectal and other cancers, promoting cell polarization and migration. FAT1 is expressed in epithelial, endothelial, and smooth muscle cells, where it regulates cell-cell interactions and responses to injury. Soluble FAT1 may serve as a potential biomarker for cancer diagnostics.
Quality Control QC Flow Cytometry Assay

Flow cytometry assay demonstrating cell surface expression of FAT1. HepG2 cells (1 × 106) were incubated with 5 µg antibody in 1 mL PBS containing 5% BSA. Antibody binding was detected with a Cy5-conjugated goat anti-rabbit secondary antibody. Shaded area: isotype control; red line: FAT1 antibody staining.

Concentration As indicated on the vial label
Formulation 80% PBS, pH 7.4; 20% glycerol; 5% trehalose; 0.04% sodium azide (preservative)
Shipping Shipped on blue ice
Storage -20 to -70 °C (long-term)
4 °C (up to one week)
For frequent use, aliquot to avoid repeated freeze-thaw cycles.