| Product Name |
Anti-IL-13RA2 Monoclonal Antibody, Recombinant |
| Catalog # |
EG-373 |
| Size |
100 µg |
| Key Features |
- Rabbit monoclonal antibody produced in HEK293 cells under serum-free, protein-free conditions
- Purified by Protein A affinity chromatography
- High batch-to-batch consistency and reproducibility
- Long-term supply security
|
| Clone Number |
M2 |
| Immunogen |
Recombinant extracellular domain of human interleukin-13 receptor alpha 2 (IL-13RA2), expressed and purified from HEK293 cells |
| Target |
Extracellular region of IL-13RA2 |
| Species Cross-Reactivity |
Human |
| Applications |
Flow Cytometry, Immunoprecipitation (IP), ELISA |
| Recommended Working Concentrations |
- Flow Cytometry / ELISA: 5 µg/mL final concentration
- Immunoprecipitation: 10 µg per 0.3–0.5 mg cell lysate
|
| Background |
Interleukin-13 receptor alpha 2 (IL-13RA2) is a high-affinity membrane receptor for interleukin-13 (IL-13), a pleiotropic Th2-derived immune regulatory cytokine. IL-13RA2 binds IL-13 with ~50-fold higher affinity than the ubiquitously expressed IL-13RA1. Under physiological conditions, IL-13 binds to IL-13RA1 and forms a heterodimeric complex with IL-4RA, activating Janus kinase (JAK) and STAT signaling. In cancer, IL-13 can bind IL-13RA2 with high affinity, independent of IL-4. IL-13RA2 is overexpressed in the majority of high-grade astrocytomas, such as glioblastoma, but not in normal brain tissue. Thus, IL-13RA2 is a promising target for glioblastoma immunotherapy, a malignancy with limited curative options. Clinical trials evaluating IL-13 fused to pseudomonas exotoxin for targeting IL-13RA2 demonstrated anti-tumor activity but were limited by side effects.
Reference:
(1) Arima, K., et al., Characterization of the interaction between interleukin-13 and interleukin-13 receptors. J Biol Chem, 2005; 280: 24915-22.
|
| Quality Control |
ELISA assay demonstrating high binding affinity of the monoclonal antibody. Calculated EC50 value: 0.05 nM.
|
| Concentration |
As indicated on the vial label |
| Formulation |
80% PBS, pH 7.4; 20% glycerol; 5% trehalose; 0.04% sodium azide (preservative) |
| Shipping |
Shipped on blue ice |
| Storage |
-20 to -70 °C (long-term)
4 °C (up to one week)
For frequent use, aliquot to avoid repeated freeze-thaw cycles.
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