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Anti-Mesothelin monoclonal antibody, recombinant

Catalog #: EG-375

$250.00

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EG-375 Anti-mesothelin-R47

Product Name	Anti-Mesothelin monoclonal antibody, recombinant
Cat #	EG-375
Size	100 µg
Key features	Rabbit monoclonal antibody produced in 293 cells with serum-free, protein-free medium, purified with protein A column; High batch-to-batch consistency and reproducibility; Long term security of supply;
Clone number	R47
Immunogen	Recombinant N-terminal mesothelin fragment (residues 296–390) that was expressed and purified from 293 cells
Reaction target	N-terminal region of mesothelin
Species Cross-Reactivity	Human
Applications	Flow Cytometry; IP; ELISA;Western blot
Working concentrations	Flow cytometry and ELISA: 5 µg/ml of final concentration is suggested. IP: 10 μg in 0.3-0.5 mg cell lysates is suggested. Western blot: 1 µg/ml.
Background	Mesothelin gene encodes a 71-kDa precursor protein that is processed to a 40-kDa protein termed mesothelin, which is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein present on the cell surface. Mesothelin is a differentiation antigen that is present on a restricted set of normal adult tissues such as the mesothelium. In contrast, it is overexpressed in a variety of cancers including ovarian cancers, non-small cell lung cancer (NSCLC), colorectal cancer (CRC) and mesothelioma. Its limited distribution on normal tissues, relatively specific expression at high levels in tumors and important role in tumor development and prognosis, make mesothelin a potential target for tumor immunotherapy.
QC	Flow cytometry assay using R47 monoclonal antibody. A431 is mesothelin negative cells. A431 (mesothelin++) is derived from A431 that was forcefully over-expressing mesothelin. One million cells were incubated with 1 μg antibody in 1 ml PBS buffer containing 5% BSA. Antibody binding was detected with Cy5-conjugated goat-anti-rabbit antibody. Blue line, isotype control antibody staining; Red line, R47 staining.
Concentration	As indicated on the vials
Formulation	80% PBS, pH 7.4; 20% Glycerol; 5% Trehalose; 0.04% Sodium azide as Preservative
Shipping	Blue ice
Storage	-20 to -70 ^oC (long term) or 4 ^oC (one week). Depending on the expected application frequency, the product can be aliquoted to avoid heavily repeated freezing/thawing cycles.

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