| Product Name |
Anti-Mesothelin Monoclonal Antibody, Recombinant |
| Catalog # |
EG-375 |
| Size |
100 µg |
| Key Features |
- Rabbit monoclonal antibody produced in HEK293 cells under serum-free, protein-free conditions
- Purified by Protein A affinity chromatography
- High batch-to-batch consistency and reproducibility
- Long-term supply security
|
| Clone Number |
R47 |
| Immunogen |
Recombinant N-terminal mesothelin fragment (residues 296–390), expressed and purified from HEK293 cells |
| Target |
N-terminal region of human mesothelin |
| Species Cross-Reactivity |
Human |
| Applications |
Flow Cytometry, Immunoprecipitation (IP), ELISA, Western Blot |
| Recommended Working Concentrations |
- Flow Cytometry / ELISA: 5 µg/mL final concentration
- Immunoprecipitation: 10 µg per 0.3–0.5 mg cell lysate
- Western Blot: 1 µg/mL
|
| Background |
The mesothelin gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein, mesothelin, present on the cell surface. Mesothelin is a differentiation antigen expressed in a restricted subset of normal adult tissues, primarily the mesothelium. In contrast, it is highly overexpressed in various malignancies, including ovarian cancer, non-small cell lung cancer (NSCLC), colorectal cancer (CRC), and mesothelioma. Due to its limited expression in normal tissues, high tumor specificity, and involvement in tumor progression and prognosis, mesothelin is considered a promising target for tumor immunotherapy.
|
| Quality Control |
Flow cytometry assay using R47 monoclonal antibody. A431 cells (mesothelin-negative) and A431 (mesothelin++), which overexpress mesothelin, were incubated with 1 µg antibody in 1 mL PBS containing 5% BSA. Antibody binding was detected with Cy5-conjugated goat anti-rabbit antibody. Blue line: isotype control; red line: R47 staining.
|
| Concentration |
As indicated on the vial label |
| Formulation |
80% PBS, pH 7.4; 20% glycerol; 5% trehalose; 0.04% sodium azide (preservative) |
| Shipping |
Shipped on blue ice |
| Storage |
-20 to -70 °C (long-term)
4 °C (up to one week)
For frequent use, aliquot to avoid repeated freeze-thaw cycles.
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