| Product Name |
E. coli Single-Stranded DNA-Binding Protein (SSB) |
| Overview |
The single-stranded DNA-binding protein (SSB) from Escherichia coli is a highly conserved, essential protein involved in all aspects of DNA metabolism, including replication, recombination, and repair. E. coli SSB functions as a homotetramer, with each subunit (~18.9 kDa) contributing to high-affinity, cooperative binding to single-stranded DNA (ssDNA). SSB protects ssDNA from nucleolytic degradation, prevents secondary structure formation, and facilitates the recruitment and activity of numerous DNA-processing enzymes. It is a prototypical model for studying protein–nucleic acid interactions and is widely used in molecular biology to enhance DNA amplification and manipulation reactions[1][2][3][4][5][6][7][8].
|
| Size |
100 µg |
| Concentration |
1 mg/mL |
| Molecular Weight |
~75.6 kDa (homotetramer; 18.9 kDa per subunit) |
| Key Features |
- Homotetrameric structure, each subunit binds ssDNA with high affinity
- Prevents reannealing and secondary structure formation in ssDNA
- Protects ssDNA from nuclease degradation
- Facilitates DNA replication, repair, and recombination
- Interacts with multiple DNA-processing enzymes via its conserved C-terminal tail
|
| Applications |
- Enhancement of PCR, isothermal amplification, and DNA sequencing reactions
- In vitro DNA replication and repair assays
- Stabilization of ssDNA in molecular biology workflows
- Model system for studying protein–nucleic acid interactions
|
| Source |
Recombinant protein expressed in E. coli |
| Quality Control |
- Purity >99% by SDS-PAGE[4]
- Functional validation in ssDNA-binding assays
- Negligible exonuclease and endonuclease contamination
|
| Formulation |
50 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5 |
| Storage |
-20 °C (long-term); avoid repeated freeze-thaw cycles |
| References |
- Meyer, R.R., Laine, P.S. The single-stranded DNA-binding protein of Escherichia coli. Microbiol Rev. 1990; 54(4):342-380.[1][2]
- Suksombat, S., et al. Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways. eLife. 2015;4:e08193.[5][6][7]
- QIAGEN. E. coli Single-Stranded DNA Binding Protein. Product Data Sheet.[4]
- Kelman, Z., et al. Molecular insights into the prototypical single-stranded DNA-binding protein of E. coli. Crit Rev Biochem Mol Biol. 2024.[8]
|