| Product Name |
Sulfolobus solfataricus Single-Stranded DNA-Binding Protein (SsoSSB) |
| Overview |
The single-stranded DNA-binding protein from Sulfolobus solfataricus (SsoSSB) is a thermostable protein that binds with high affinity and specificity to single-stranded DNA (ssDNA). SsoSSB plays a critical role in DNA replication, recombination, and repair in hyperthermophilic archaea. It protects ssDNA from nucleolytic degradation, prevents secondary structure formation, and facilitates the action of DNA polymerases and helicases at elevated temperatures. SsoSSB is widely used in molecular biology applications requiring robust ssDNA stabilization under extreme conditions.
|
| Size |
100 µg |
| Concentration |
1 mg/mL |
| Molecular Weight |
~16.3 kDa (monomer) |
| Key Features |
- Thermostable: active at temperatures up to 80–90 °C
- Binds ssDNA with high affinity and specificity
- Prevents formation of secondary structures in ssDNA
- Facilitates DNA replication, repair, and recombination in high-temperature environments
- Functions as a homotetramer, similar to bacterial SSBs
|
| Applications |
- Thermostable DNA amplification (PCR, isothermal amplification)
- In vitro DNA replication and repair assays at high temperature
- Stabilization of ssDNA in molecular biology and biotechnology workflows
- Model system for studying DNA-protein interactions in archaea
|
| Source |
Recombinant protein expressed in E. coli |
| Quality Control |
- Purity >95% by SDS-PAGE
- Functional validation in ssDNA-binding assays
|
| Formulation |
20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5 |
| Storage |
-80 °C (long-term); avoid repeated freeze-thaw cycles |
| References |
- Robbins, J.B., et al. Structural and thermodynamic studies of the ssDNA-binding protein from Sulfolobus solfataricus. J Mol Biol. 2004; 340(4): 647–658.
- Kelly, T.J., et al. Archaeal SSBs: Structure, function and applications. Extremophiles. 2010; 14(1): 1–8.
- Richard, D.J., et al. Single-stranded DNA binding proteins from Sulfolobus solfataricus and Sulfolobus acidocaldarius. J Bacteriol. 2001; 183(19): 6075–6083.
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