| Description |
T4 Gene 32 Protein is an essential single-stranded DNA (ssDNA) binding protein that plays multiple roles in bacteriophage T4 DNA metabolism. Key characteristics include:
- Molecular Weight: 33.5 kDa (monomeric form)
- Binding Capacity: Binds cooperatively to ssDNA with a stoichiometry of 1 protein per 7-10 nucleotides
- Thermostability: Remains active up to 45°C
- Functional Domains: Contains distinct ssDNA binding and protein interaction domains
- Buffer Compatibility: Active in most common molecular biology buffers (pH 6.5-8.5)
|
| Size |
100 µg |
| Concentration |
1 mg/mL |
| Applications |
- Nucleic Acid Manipulation:
- Stabilization of ssDNA structures for electron microscopy
- Prevention of ssDNA secondary structure formation
- Enhancement of DNA hybridization efficiency
- Enzyme Reactions:
- Increase yield and processivity in RT-PCR (0.1-1 μg/reaction)
- Enhance T4 DNA polymerase activity (1:1 molar ratio with polymerase)
- Improve PCR specificity and yield (0.5-2 μg/50 μL reaction)
- Facilitate restriction enzyme digestion of problematic templates
|
| Source |
Recombinantly expressed in E. coli BL21(DE3) containing the bacteriophage T4 gene 32 expression construct |
| Formulation |
T4 Gene 32 Protein: 10 mg/mL in storage buffer (10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5 @ 25°C)
|
| Quality Control |
- Tested for absence of endonuclease, exonuclease, and phosphatase activities
- Verified ssDNA binding activity by electrophoretic mobility shift assay
- Confirmed >95% purity by SDS-PAGE analysis
- Functionally validated in PCR enhancement assays
|
| Storage Conditions |
- Short-term: -20°C (stable for 12 months)
- Long-term: -80°C (stable for 24 months)
- Note: Aliquot to avoid repeated freeze-thaw cycles
|
| Recommended Usage |
Typical working concentrations:
- PCR/RT-PCR: 0.1-1 μg per 50 μL reaction
- ssDNA stabilization: 1-2 μg per μg DNA
- Restriction enzyme enhancement: 0.5-1 μg per reaction
|