| Product Name |
Thermus aquaticus MutS DNA Mismatch Repair Protein (6×His-Tagged) |
| Catalog Number |
[Insert catalog number] |
| Size |
100 µg (1 mg/mL stock concentration) |
| Description |
The Thermus aquaticus MutS protein is a thermostable DNA mismatch repair enzyme that specifically recognizes and binds to:
- All single base mismatches (GT, CT, AG, etc.)
- Insertion/deletion loops (1-4 bases)
- Unpaired bases in heteroduplex DNA
Key Features:
- Broad temperature range (0-75°C) with optimal activity at 65°C
- Thermostable ATPase activity (kcat = 0.5 min-1 at 65°C)
- C-terminal 6×His tag for easy purification
- >95% purity by SDS-PAGE analysis
- Compatible with multiple detection methods (gel shift, bead capture, etc.)
|
| Size |
100 µg |
| Concentration |
1 mg/mL |
| Applications |
- Gene synthesis QC: Error correction in PCR assemblies
- Mutation detection: SNP and indel identification
- Isothermal diagnostics: Rapid SNP detection without thermal cycling
- DNA analysis: Heteroduplex characterization
|
| Source |
Recombinant expression in E. coli |
| Protein Characteristics |
- Molecular Weight: 92.8 kDa (theoretical)
- Extinction Coefficient: 99,430 M-1cm-1 (280 nm)
- Tag: C-terminal 6×His
- Accession #: AAC43637 (NCBI)
|
| Protein Sequence |
1 MKIEHMEGML KGEGPGPLPP LLQQYVELRD QYPDYLLLFQ VGDFYECFGE DAERLARALG
61 LVLTHKTSKD FTTPMAGIPL RAFEAYAERL LKMGFRLAVA DQVEPAEEAE GLVRREVTQL
121 LTPGTLLQES LLPREANYLA AIATGDGWGL AFLDVSTGEF KGTVLKSKSA LYDELFRHRP
181 AEVLLAPELL ENGAFLDEFR KRFPMVLSEA PFEPEGEGPL ALRRARGALL AYAQRTQGGA
241 LSLQPFRFYD PGAFMRLPEA TLRALEVFEP LRGQDTLFSV LDETRTAPGR RLLQSWLRHP
301 LLDRGPLEAR LDRVEGFVRE GALREGVRRL LYRLADLERL ATRLELGRAS PKDLGALRRS
361 LQILPELRAL LGEEVGLPDL SPLKEELEAA LVEDPPLKVS EGGLIREGYD PDLDALRAAH
421 REGVAYFLEL EERERERTGI PTLKVGYNAV FGYYLEVTRP YYERVPKEYR PVQTLKDRQR
481 YTLPEMKEKE REVYRLEALI RRREEEVFLE VRERAKRQAE ALREAARILA ELDVYAALAE
541 VAVRYGYVRP RFGDRLQIRA GRHPVVERRT EFVPNDLEMA HELVLITGPN MAGKSTFLRQ
601 TALIALLAQV GSFVPAEEAH LPLFDGIYTR IGASDDLAGG KSTFMVEMEE VALILKEATE
661 NSLVLLDEVG RGTSSLDGVA IATAVAEALH ERRAYTLFAT HYFELTALGL PRLKNLHVAA
721 REEAGGLVFY HQVLPGPASK SYGVEVAAMA GLPKEVVARA RALLQAMAAR REGALDAVLE
781 RLLALDPDRL TPLEALRLLQ ELKALALGAP LDTMKGKLAA ALEHHHHHH
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| Buffers
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