| Product Name |
T7 Phage Single-Stranded DNA-Binding Protein (gp2.5) |
| Overview |
The T7 phage single-stranded DNA-binding protein, also known as gene 2.5 protein (gp2.5), is an essential ssDNA-binding protein from bacteriophage T7. It plays a central role in DNA replication, recombination, repair, and viral genome maturation. Gp2.5 binds strongly and specifically to single-stranded DNA, removing secondary structures and facilitating the action of T7 DNA polymerase and helicase during DNA synthesis and strand displacement. Its homologous DNA annealing activity is crucial for recombination and concatemer formation in T7 DNA metabolism[1][2][3][6][8][9].
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| Molecular Weight |
~25–31 kDa (monomer) |
| Size |
100 µg |
| Key Features |
- Binds single-stranded DNA with high affinity and specificity
- Promotes DNA replication, recombination, and repair in T7 phage systems
- Facilitates annealing of homologous ssDNA and formation of concatemeric DNA
- Physically interacts with T7 DNA polymerase and primase-helicase to coordinate replication fork progression
- Contains a conserved OB-fold for ssDNA interaction and an acidic C-terminal tail required for protein-protein interactions
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| Applications |
- In vitro DNA replication assays with T7 DNA polymerase
- Studies of DNA recombination and repair mechanisms
- Enhancement of strand displacement and lagging strand synthesis in T7-based systems
- General use as a model ssDNA-binding protein in molecular biology research
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| Source |
Recombinant protein expressed in E. coli |
| Quality Control |
- Purity >95% by SDS-PAGE
- Functional validation in ssDNA-binding and DNA annealing assays
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| Formulation |
Supplied in 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5 |
| Storage |
-80 °C (long-term); avoid repeated freeze-thaw cycles |
| References |
- Pavletich, N.P., et al. Structure of the gene 2.5 protein, a single-stranded DNA binding protein from bacteriophage T7. PNAS 98, 6052–6057 (2001).[1]
- Boehmer, P.E., et al. Gp2.5, the multifunctional bacteriophage T7 single-stranded DNA binding protein. Genes 9, 201 (2018).[2][6]
- Qimron, U., et al. A single-stranded DNA-binding protein of bacteriophage T7 promotes DNA annealing. J Biol Chem 278, 29538–29545 (2003).[3]
- Willingham, S.B., et al. C-terminal phenylalanine of bacteriophage T7 single-stranded DNA-binding protein is essential for strand displacement synthesis by T7 DNA polymerase. Nucleic Acids Res 37, 7859–7867 (2009).[8]
- Chase, J.W., et al. A DNA-binding protein induced by bacteriophage T7. J Biol Chem 248, 6953–6962 (1973).[9]
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