| Description | GRGreen Loading Buffer is a 6X gel loading buffer containing GRGreen Nucleic Acid Stain, a highly sensitive fluorescent dye for instant DNA band visualization during or after agarose gel electrophoresis. The dye forms a tight complex with nucleic acids, comigrates with your samples, and enables direct viewing on standard UV or blue light transilluminators—no post-staining or destaining required. |
| Key Benefits | - Instant visualization of DNA bands—no additional staining steps
- Prestaining improves band sharpness and resolution, eliminating smearing and "smiley" effects
- Compatible with both UV and blue light detection systems
- Dye can be completely removed by alcohol precipitation or column purification
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| Applications | - Routine agarose gel electrophoresis of DNA samples
- Visualization of DNA markers and ladders
- Sample preparation for downstream DNA purification
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| Protocol | Add 2 µl of 6X GRGreen Loading Buffer to 10 µl DNA sample or marker. Load and run the gel as usual. Visualize DNA bands using a UV or blue light transilluminator. |
| Size | 1 ml |
| Storage & Handling | Store at -20 °C, protected from light. Stable for 12 months at -20 °C and 6 months at 4 °C. |
| Additional Information | - Supplied as a 6X loading buffer
- Compatible with standard agarose gels and electrophoresis protocols
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