| Description |
Fast Electrophoresis Buffer (FEB Buffer) is a proprietary blend of sodium acetate, sodium borate, and boric acid, optimized for rapid and high-resolution separation of DNA on agarose or polyacrylamide gels. FEB Buffer enables higher voltage operation, allowing for rapid run times (10–30 minutes) and sharp, well-resolved DNA bands.
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| Key Features |
- Enables electrophoresis at higher voltages (120–250 V)
- Rapid DNA separation: 10–30 minute run times
- High-resolution band separation on agarose or polyacrylamide gels
- Compatible with GRGreen and Ethidium Bromide DNA stains
- Suitable for downstream DNA purification (e.g., Qiagen Gel Purification Kit)
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| Preparation & Usage |
- To prepare 1X FEB running buffer, dilute 100 ml of 20X FEB with 900 ml ddH2O.
- Recommended voltage: 120 V for small chambers; 200–250 V for mid to large apparatus (optimize as needed).
- Prepare agarose gels using 1X FEB Buffer for best results.
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| Compatibility |
- Compatible with GRGreen and Ethidium Bromide staining
- Compatible with downstream gel extraction and purification kits
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| Performance Example |
Gel image: 100 bp ladder and 1 kb ladder separated using FEB buffer, stained with GRGreen.
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