| Product Name |
TAT-NLS-Cas9 Nuclease (6×His-tagged) |
| Size |
100 µg (1 mg/mL stock concentration) |
| Description |
Recombinant CRISPR-Cas9 nuclease engineered for enhanced cellular delivery and nuclear localization. This protein contains:
- N-terminal modifications:
- TAT cell-penetrating peptide (GRKKRRQRRRPPAGTSV)
- SV40 nuclear localization signal (KKKRKV)
- C-terminal modifications:
- SV40 nuclear localization signal
- 6×His tag for purification
- Source: Purified from codon-optimized E. coli expression system
|
| Applications |
- Genome editing:
- Cell culture transfection
- One-cell embryo microinjection
- Organoid engineering
- In vitro applications:
- Targeted DNA cleavage assays
- Protospacer adjacent motif (PAM) validation
- gRNA activity testing
|
| Specifications |
- Molecular Weight: ~160 kDa (theoretical)
- Purity: >95% (SDS-PAGE with Coomassie staining)
- Storage Buffer: 10 mM HEPES (pH 7.4), 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol
- Activity: >90% target cleavage efficiency with validated gRNAs
|
| Stability |
- -80°C: 2 years
- -20°C: 1 year
- 4°C: 1 month
|
| Recommended Usage |
For genome editing in cultured cells:
- Complex with gRNA (molar ratio 1:2) for 10 min at room temperature
- Deliver using appropriate transfection method
- Typical working concentration: 0.5-2 µM
|
| Shipping |
Dry ice (stable for 72 hours in transit) |
| Storage |
-20°C (short-term), -80°C (long-term) |