A polycistronic mRNA cocktail encoding the four Yamanaka factors (KLF4, OCT4, SOX2, c-MYC) plus LIN28A and RFP reporter for human cellular reprogramming. The RFP reporter enables real-time monitoring of transfection efficiency.

Key Features:

• Expresses all essential reprogramming factors in a single formulation
• RFP reporter (Ex/Em 558/583 nm) for transfection monitoring
• Compatible with standard TRITC filter sets
• Optimized for high-efficiency iPSC generation

• Generation of integration-free induced pluripotent stem cells (iPSCs)
• Human somatic cell reprogramming
• Stem cell research and regenerative medicine applications

• 5' Capping: ARCA (Cap 0) or CleanCap AG (Cap 1)
• Nucleotide Modifications:
  • N1-methyl-pseudouridine (Ψ)
  • 5-methylcytidine (5mC)
• 3' Tail: 180 nt poly(A) sequence
• Sequence Optimization: Codon-optimized for human cells

• Concentration: 1.0 ± 0.2 µg/µL (A₂₆₀)
• Purity: >90% full-length by denaturing agarose gel
• Endotoxin: <0.1 EU/µg
• Recommended Storage: -80°C (aliquots recommended)

For human fibroblast reprogramming:

1. Plate 5×10⁴ cells/well in 6-well plate
2. Transfert with 1-2 µg mRNA cocktail using Lipofectamine MessengerMAX™
3. Repeat transfections daily for 12-16 days
4. Monitor RFP expression to assess transfection efficiency
5. Pick iPSC colonies at day 18-21

Full protocol: MessengerMAX™ Protocol

• Cap integrity verified by RNase H assay
• Poly(A) length confirmed by Northern blot
• Functional validation in reprogramming assays

Mandal & Rossi. (2013) Reprogramming human fibroblasts to pluripotency using modified mRNA. Nature Protocols 8:568-582. doi:10.1038/nprot.2013.019