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This self-amplifying mRNA encodes four
nonstructural proteins (nsP1 to nsP4) derived from a positive-stranded
alphavirus venezuelan equine encephalitis virus (VEEV), and Oplophorus (OLuc) fused to EGFP with T2A self-cleaving peptides.
OLuc is an engineered enzyme from a 19 kDa subunit of luciferase from a deep sea shrimp Oplophorus gracilirostris, it is ~ 150 times more sensitive than traditional firefly luciferase. It uses furimazine as substrate for bioluminescence assay.
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