Genome Engineering
Product 9/9
Previous Return to the Product List Next
Return to the Product List

Human Klf4-Oct4-Sox2-c-Myc (KOSM) mRNA

Cat #: EG-308

   
Qty   
Product Name Human Klf4-Oct4-Sox2-c-Myc (KOSM) mRNA
Description

This mRNA will express 4 human transcription factors, OCT4, KLF4, c-MYC, SOX2, LIN28A and RFP. The delivery efficiency can be monitored by fluorescence microscope using these Omega Optical filter sets: QMAX-Yellow, XF108-2, XF101-2, and XF111-2, or TRITC filter
Applications Reprogramming human fibroblasts to generate induced pluripotent stem (iPS) cells
Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Concentration ~1 µg/µl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 oC
References Reprogramming human fibroblasts to pluripotency using modified mRNA. Mandal & Rossi, Nature Protocols 8,568-582 (2013) | doi:10.1038/nprot.nprot.2013.019.