| Description |
Taq DNA Polymerase is purified from E.coli cells with a cloned pol gene from Thermus aquaticus YT1. The purified enzyme is a monomeric protein with molecular weight of 94 kDa.The enzyme catalyzes 5'->3' synthesis of DNA, has no detectable 3'->5' exonuclease (proofreading) activity and possesses low 5'->3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products.
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| Features |
- Thermostable – half life is more than 40 min at 95°C.
- Generates PCR products with 3’-dA overhangs.
- Purity (SDS-PAGE): >99%
- Contaminants: E.coli 16S rDNA is below detection limit by RT-PCR
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| Applications |
Routine PCR amplification of up to 5 kb DNA fragment. |
| Source |
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 |
| Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. |
| Components |
- Recombinant Taq DNA polymerase: 5 units/µL in 20 mM Tris-HCl (pH 7.5), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, Stabilizer and 50% (v/v) glycerol.
- 10X Standard Taq Buffer: 100 mM Tris-HCl, 500 mM KCl, 15 mM MgCl2, pH 8.3 @ 25°C
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| Storage Condition |
-20°C.
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