| Service Name |
Yeast Expression and Recombinant Protein Purification Service (≥3 mg) |
| Description |
Our Yeast Expression and Recombinant Protein Purification Service leverages over 15 years of scientific expertise and state-of-the-art technologies to deliver high-quality recombinant proteins. Using advanced yeast expression systems and AKTA FPLC chromatography, we produce and purify proteins with high yield and purity—ideal for research, assay development, or structural biology. We offer flexible project initiation via plasmid, gene synthesis, or cDNA clone sourcing, and support parallel optimization and high-throughput screening for challenging targets.
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| Key Features & Advantages |
- Highly experienced team (average 15+ years in molecular biology and protein science)
- Comprehensive workflow: cloning, expression, purification, and QC
- Advanced AKTAExplore and AKTAPurifier FPLC systems with a wide range of columns (affinity, ion exchange, hydrophobic, size exclusion, desalting)
- Parallel optimization and high-throughput screening for expression and solubility
- Flexible input: plasmid, gene synthesis, or cDNA clone sourcing
- Scalable: milligram to gram quantities
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| Workflow & Timeline |
- Cloning and Subcloning (2 weeks):
- PCR amplification with high-fidelity polymerase
- Cloning into yeast expression vector
- Restriction digestion analysis and sequencing
- Plasmid DNA preparation
- Pilot Expression and Purification (1–2 weeks):
- Yeast transformation and small-scale expression
- Pilot purification and QC (SDS-PAGE, dot-blot, or Western blot)
- Yield estimation
- Large-Scale Expression and Purification (1–2 weeks):
- Large-scale yeast culture and protein expression
- Purification using AKTA FPLC (affinity, IEX, SEC, HIC, desalting as needed)
- Dialysis, concentration, and QC (SDS-PAGE, Western blot, protein quantification)
- Delivery of purified, ready-to-use protein
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| Deliverables |
- ≥3 mg purified recombinant protein (>95% purity)
- Quality control data (SDS-PAGE, Western blot, yield)
- Chromatograms and process documentation
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| Required Materials |
- 5 µg plasmid containing gene of interest (with complete vector sequence in electronic format)
- or protein sequence for gene synthesis (additional fee applies)
- or accession number for cDNA sourcing (e.g., ATCC, Origene, GeneCopoeia)
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| Applications |
- Structural biology and biophysical studies
- Enzyme assays and functional analysis
- Antibody production and immunization
- Assay development and screening
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Quotation & Ordering |
- Request a quotation form from customer service: crm@excellgen.com
- Submit the completed form and PO/credit card info by email, fax, or phone
- Ship samples with a hard copy of the completed form to:
Protein Services, Excellgen, 847 Quince Orchard Blvd, Suite H, Gaithersburg, MD, 20878, US
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