| Service Name |
Protein Expression and Purification using Baculovirus Expression System |
| Description |
Our Baculovirus Expression Service offers high-yield, scalable production and purification of recombinant proteins using the baculovirus-insect cell system. We generate high-titer baculovirus stocks (up to 108 pfu) and can deliver milligram to gram quantities of active, purified protein within 6–8 weeks. This platform is ideal for producing eukaryotic proteins requiring proper folding and post-translational modifications.
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| Key Features & Advantages |
- High-titer baculovirus generation and amplification
- Milligram to gram scale protein production
- Advanced AKTA FPLC purification (affinity, ion exchange, size exclusion, etc.)
- Proteins with native folding and post-translational modifications
- Fast turnaround: 6–8 weeks from cloning to purified protein
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| Workflow & Timeline |
- Cloning and Subcloning (1–2 weeks):
- PCR amplification and cloning into baculovirus expression vector
- Sequence verification and plasmid preparation
- Bacmid Generation (1 week):
- Transformation of DH10Bac cells and selection of recombinant bacmids
- Bacmid DNA purification
- Baculovirus Generation & Amplification (2–3 weeks):
- Transfection of insect cells and plaque purification
- Baculovirus amplification and titer determination
- Protein Expression (1 week):
- Infection of insect cells and protein expression
- Harvesting of cells and/or supernatant
- Protein Purification (1 week):
- Affinity and AKTA FPLC purification
- Dialysis, concentration, and QC (SDS-PAGE, Western blot, protein quantitation)
- Delivery of purified, ready-to-use protein
Total time required: 6–8 weeks
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| Deliverables |
- ≥3 mg purified recombinant protein (>95% purity)
- Quality control data (SDS-PAGE, Western blot, yield)
- Chromatograms and process documentation
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| Required Materials |
- 5 µg plasmid containing gene of interest (with complete vector sequence in electronic format)
- or protein sequence for gene synthesis (additional fee applies)
- or accession number for cDNA sourcing
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| Applications |
- Production of eukaryotic proteins requiring complex folding or modifications
- Enzyme, antibody, and vaccine research
- Structural biology and functional studies
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Quotation & Ordering |
- Request a quotation form from customer service: crm@excellgen.com
- Submit the completed form and PO/credit card info by email, fax, or phone
- Ship samples with a hard copy of the completed form to:
Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743
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