| Product Name |
Anti-FAT1 monoclonal
antibody, recombinant |
| Cat # |
EG-371 |
| Size |
100 µg |
| Key features |
Rabbit monoclonal antibody
produced in 293 cells with serum-free, protein-free medium, purified
with protein A column;
High batch-to-batch consistency and reproducibility;
Long term security of supply; |
| Clone number |
M5 |
| Immunogen |
Recombinant extracellular
FAT1 fragment close to transmembrane domain, which was expressed and
purified from 293 cells |
| Reaction
target |
Extracellular region that
is close to the transmembrane domain |
| Species
Cross-Reactivity |
Human |
| Applications |
Flow Cytometry; IP; ELISA; |
| Working
concentrations |
Flow cytometry and ELISA: 5
µg/ml of final concentration is suggested.
IP: 10 µg in 0.3-0.5 mg cell lysates is suggested.
|
| Background |
Procadherin FAT1 is a
gigantic single pass transmembrane protein with the extracellular
portion consisting of 34 cadherin repeats, 5 EGF-like domains and a
laminin-G like domain. The intracellular part has an
nuclear-translocation signal and can regulate specific gene expression
after being activated. FAT1 regulates Hippo, TGF-beta, and
Wnt-beta-catenin signaling in colorectal and other cancers, promoting
cell polarization and migration. Procadherin FAT1 is expressed in
epithelial, endothelial and smooth muscle cells and modulates cell-cell
contact interactions in their response to injury. Soluble FAT1 may be a
biomarker candidate cancer diagnostics. |
| QC |
Flow cytometry assay showing cell surface expression of
FAT1. HepG2 cells (10^6) were incubated with 5 µg antibody in 1 ml PBS
buffer containing 5% BSA. Antibody binding was detected with
Cy5-conjugated goat-anti-rabbit antibody. Shaded area, isotype control
antibody staining; red line, FAT1 antibody staining.
|
| Concentration |
As indicated on the vials |
| Formulation |
80% PBS, pH 7.4; 20%
Glycerol; 5% Trehalose; 0.04% Sodium azide as Preservative. |
| Shipping |
Blue ice |
| Storage |
-20 to -70 oC
(long term)
or 4 oC (one week).
Depending on the expected frequency of use, the product can be
aliquoted to avoid heavily repeated freezing/thawing cycles. |