| Cat # |
EG-325
|
| Description |
A recombinant form of the full length
spike glycoprotein from severe acute respiratory syndrome-related
coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced
in HEK293 cells and purified by nickel affinity and ion exchange
chromatography. This recombinant protein has been stabilized by several
mutations: removing the polybasic S1/S2 cleavage site by changing RRAR
to GSAS (residues 682 to 685), KV to PP mutations (residues 986 and
987), and 4 additional proline substitutions, producing a more stable
HexaPro variant (K986P, V987P, F817P, A892P, A899P and A942P). T4
foldon
trimerization domain and Octa-histidine tag is located at the
C-terminus.
This protein forms a trimer, which is suitable for screening of
neutralizing antibodies in vitro |
Mutations
|
R682G, R683S, A684A, R685S,
K986P, V987P, F817P, A892P, A899P and A942P |
Tags
|
C-terminal Octa-histidine and Twin-Strep tags |
Purification
|
FPLC by nickel
affinity and ion exchange chromatography |
| Sequence |
1 CVNLTTRTQL PPAYTNSFTR GVYYPDKVFR SSVLHSTQDL FLPFFSNVTW
51 FHAIHVSGTN GTKRFDNPVL PFNDGVYFAS TEKSNIIRGW IFGTTLDSKT
101 QSLLIVNNAT NVVIKVCEFQ FCNDPFLGVY YHKNNKSWME SEFRVYSSAN
151 NCTFEYVSQP FLMDLEGKQG NFKNLREFVF KNIDGYFKIY SKHTPINLVR
201 DLPQGFSALE PLVDLPIGIN ITRFQTLLAL HRSYLTPGDS SSGWTAGAAA
251 YYVGYLQPRT FLLKYNENGT ITDAVDCALD PLSETKCTLK SFTVEKGIYQ
301 TSNFRVQPTE SIVRFPNITN LCPFGEVFNA TRFASVYAWN RKRISNCVAD
351 YSVLYNSASF STFKCYGVSP TKLNDLCFTN VYADSFVIRG DEVRQIAPGQ
401 TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF
451 ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS
501 FELLHAPATV CGPKKSTNLV KNKCVNFNFN GLTGTGVLTE SNKKFLPFQQ
551 FGRDIADTTD AVRDPQTLEI LDITPCSFGG VSVITPGTNT SNQVAVLYQD
601 VNCTEVPVAI HADQLTPTWR VYSTGSNVFQ TRAGCLIGAE HVNNSYECDI
651 PIGAGICASY QTQTNSPGSA SSVASQSIIA YTMSLGAENS VAYSNNSIAI
701 PTNFTISVTT EILPVSMTKT SVDCTMYICG DSTECSNLLL QYGSFCTQLN
751 RALTGIAVEQ DKNTQEVFAQ VKQIYKTPPI KDFGGFNFSQ ILPDPSKPSK
801 RSPIEDLLFN KVTLADAGFI KQYGDCLGDI AARDLICAQK FNGLTVLPPL
851 LTDEMIAQYT SALLAGTITS GWTFGAGPAL QIPFPMQMAY RFNGIGVTQN
901 VLYENQKLIA NQFNSAIGKI QDSLSSTPSA LGKLQDVVNQ NAQALNTLVK
951 QLSSNFGAIS SVLNDILSRL DPPEAEVQID RLITGRLQSL QTYVTQQLIR
1001 AAEIRASANL AATKMSECVL GQSKRVDFCG KGYHLMSFPQ SAPHGVVFLH
1051 VTYVPAQEKN FTTAPAICHD GKAHFPREGV FVSNGTHWFV TQRNFYEPQI
1101 ITTDNTFVSG NCDVVIGIVN NTVYDPLQPE LDSFKEELDK YFKNHTSPDV
1151 DLGDISGINA SVVNIQKEID RLNEVAKNLN ESLIDLQELG KYEQGSGYIP
1201 EAPRDGQAYV RKDGEWVLLS TFLGRSLEVL FQGPGHHHHH HHHSAWSHPQ
1251 FEKGGGSGGG GSGGSAWSHP QFEK
|
Mol Wt
|
140.7kDa |
Source
|
HEK 293 |
Purity
|
90~95% by SDS-PAGE |
|
Applications
|
Screening of neutralizing antibodies, western blot, ELISA, animal
vaccination
|
| Storage Buffer |
PBS + 50% Glycerol
|
| Storage |
-20ºC ~ -80ºC |
| Shipping |
dry ice |
References
|
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