| Product Name |
SARS-CoV-2 Spike Glycoprotein, Stabilized Trimer, His Tag |
| Catalog Number |
EG-303 |
| Description |
Recombinant full-length SARS-CoV-2 spike glycoprotein (Wuhan-Hu-1, GenBank: QHD43416.1) expressed in HEK293 cells, stabilized as a trimer with mutations (RRAR682–685A, K986P, V987P) and a C-terminal T4 foldon trimerization domain followed by a 6xHis tag. Purified by FPLC using nickel affinity and ion exchange chromatography. |
| Mutations |
RRAR682–685A, K986P, V987P |
| Tags |
C-terminal 6xHis |
| Purification |
FPLC (nickel affinity and ion exchange chromatography) |
| Sequence |
CVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTN 60
GTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQ 120
FCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVF 180
KNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDS 240
SSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQ 300
TSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASF 360
STFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVI 420
AWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQS 480
YGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTES 540
NKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGV 600
NCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQ 660
TQTNSPASVASQSIIAYTMSLGAENSVAYSNN SIAIPTNFTISVTTEILPVSMTKTSVDC 720
TMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFG 780
GFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGL 840
TVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYE 900
NQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLN 960
DILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSK 1020
RVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSN 1080
GTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKN 1140
HTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPSGRLV 1200
PRGSPGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGHHHHHH 1237
|
| Molecular Weight |
137.6 kDa (theoretical) |
| Source |
HEK293 cells |
| Purity |
≥90% by SDS-PAGE |
| Applications |
Screening neutralizing antibodies, Western blot, ELISA, animal immunization studies |
| Storage Buffer |
PBS with 50% glycerol |
| Storage |
-20°C to -80°C |
| Shipping |
Dry ice |
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