| Cat # |
EG-328
|
| Description |
A recombinant form of
spike glycoprotein S2 extracellular domain (ECD, S686 to P1213) from severe acute respiratory syndrome-related
coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced
in HEK293 cells and purified by nickel affinity and ion exchange
chromatography.
|
Mutations
|
WT |
Tags
|
C-terminal hexa-histidine |
Purification
|
FPLC by nickel
affinity and ion exchange chromatography |
| Sequence |
1 SVASQSIIAY TMSLGAENSV AYSNNSIAIP TNFTISVTTE ILPVSMTKTS
51 VDCTMYICGD STECSNLLLQ YGSFCTQLNR ALTGIAVEQD KNTQEVFAQV
101 KQIYKTPPIK DFGGFNFSQI LPDPSKPSKR SFIEDLLFNK VTLADAGFIK
151 QYGDCLGDIA ARDLICAQKF NGLTVLPPLL TDEMIAQYTS ALLAGTITSG
201 WTFGAGAALQ IPFAMQMAYR FNGIGVTQNV LYENQKLIAN QFNSAIGKIQ
251 DSLSSTASAL GKLQDVVNQN AQALNTLVKQ LSSNFGAISS VLNDILSRLD
301 KVEAEVQIDR LITGRLQSLQ TYVTQQLIRA AEIRASANLA ATKMSECVLG
351 QSKRVDFCGK GYHLMSFPQS APHGVVFLHV TYVPAQEKNF TTAPAICHDG
401 KAHFPREGVF VSNGTHWFVT QRNFYEPQII TTDNTFVSGN CDVVIGIVNN
451 TVYDPLQPEL DSFKEELDKY FKNHTSPDVD LGDISGINAS VVNIQKEIDR
501 LNEVAKNLNE SLIDLQELGK YEQYIKWPAH HHHHHH
|
Mol Wt
|
59.0 kDa |
Source
|
HEK 293 |
Purity
|
90~95% by SDS-PAGE |
|
Applications
|
Screening of neutralizing antibodies, western blot, ELISA, animal
vaccination
|
| Storage Buffer |
PBS + 50% Glycerol
|
| Storage |
-20ºC ~ -80ºC |
| Shipping |
dry ice |
References
|
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