| Product Name |
SARS-CoV-2 Spike Glycoprotein, HexaPro Variant, His-Strep Tag |
| Catalog Number |
EG-325 |
| Description |
Recombinant full-length SARS-CoV-2 spike glycoprotein (Wuhan-Hu-1, GenBank: QHD43416.1) expressed in HEK293 cells, stabilized as a trimer (HexaPro variant) with mutations (RRAR682–685GSAS, F817P, A892P, A899P, A942P, K986P, V987P) and a C-terminal T4 foldon trimerization domain followed by 8xHis and Twin-Strep tags. Purified by FPLC using nickel affinity and ion exchange chromatography. |
| Mutations |
RRAR682–685GSAS, F817P, A892P, A899P, A942P, K986P, V987P |
| Tags |
C-terminal 8xHis and Twin-Strep |
| Purification |
FPLC (nickel affinity and ion exchange chromatography) |
| Sequence |
CVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTN 60
GTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQ 120
FCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVF 180
KNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDS 240
SSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQ 300
TSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASF 360
STFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVI 420
AWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQS 480
YGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTES 540
NKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDV 600
NCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQ 660
TQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNN SIAIPTNFTISVTTEILPVSMTKTS 720
VDC TMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIK 780
DFGGFNFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKF 840
NGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGPALQIPFPMQMAYRFNGIGVTQNV 900
LYENQKLIANQFNSAIGKIQDSLSSTPSALGKLQDVVNQNAQALNTLVKQLSSNFGAISS 960
VLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLG 1020
QSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVF 1080
VSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKY 1140
FKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQGSGYIPE 1200
APRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGS 1260
GGSAWSHPQFEK 1273
|
| Molecular Weight |
140.7 kDa (theoretical) |
| Source |
HEK293 cells |
| Purity |
≥90% by SDS-PAGE |
| Applications |
Screening neutralizing antibodies, Western blot, ELISA, animal immunization studies |
| Storage Buffer |
PBS with 50% glycerol |
| Storage |
-20°C to -80°C |
| Shipping |
Dry ice |
|