Product Name |
Thermus aquaticus MutS DNA mismatch repair protein |
Description |
The Taq MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This Muts protein binds in vitro to heteroduplex DNAs containing mispaired or unpaired bases over a wide temperature range from 4 to 70 °C and has a thermostable ATPase activity. This thermostable Taq MutS is active at temperature between 0 to 75°C. Since Taq MutS efficiently binds to 1-4 bases deletion (or insertion) and mismatch base pairs of GT, CT and AG, it is useful for detecting these mutations. Mutations can be detected in polyacrylamide gels |
Applications |
- Remove mismatch DNA (error correction) from gene synthesis reaction
- Mutation detection and removal
- Rapid isothermal SNP detection
|
Source |
E. coli |
Fusion Tag |
None |
Purification Method |
FPLC |
Purity |
~ 95% as determined by SDS-PAGE
|
Accession # |
AAC43637, TAU3311, U33117 |
Gene Name |
Thermus aquaticus MutS DNA mismatch repair protein |
Protein Sequence |
1 MEGMLKGEGP GPLPPLLQQY VELRDQYPDY LLLFQVGDFY ECFGEDAERL ARALGLVLTH
61 KTSKDFTTPM AGIPLRAFEA YAERLLKMGF RLAVADQVEP AEEAEGLVRR EVTQLLTPGT
121 LLQESLLPRE ANYLAAIATG DGWGLAFLDV STGEFKGTVL KSKSALYDEL FRHRPAEVLL
181 APELLENGAF LDEFRKRFPV MLSEAPFEPE GEGPLALRRA RGALLAYAQR TQGGALSLQP
241 FRFYDPGAFM RLPEATLRAL EVFEPLRGQD TLFSVLDETR TAPGRRLLQS WLRHPLLDRG
301 PLEARLDRVE GFVREGALRE GVRRLLYRLA DLERLATRLE LGRASPKDLG ALRRSLQILP
361 ELRALLGEEV GLPDLSPLKE ELEAALVEDP PLKVSEGGLI REGYDPDLDA LRAAHREGVA
421 YFLELEERER ERTGIPTLKV GYNAVFGYYL EVTRPYYERV PKEYRPVQTL KDRQRYTLPE
481 MKEKEREVYR LEALIRRREE EVFLEVRERA KRQAEALREA ARILAELDVY AALAEVAVRY
541 GYVRPRFGDR LQIRAGRHPV VERRTEFVPN DLEMAHELVL ITGPNMAGKS TFLRQTALIA
601 LLAQVGSFVP AEEAHLPLFD GIYTRIGASD DLAGGKSTFM VEMEEVALIL KEATENSLVL
661 LDEVGRGTSS LDGVAIATAV AEALHERRAY TLFATHYFEL TALGLPRLKN LHVAAREEAG
721 GLVFYHQVLP GPASKSYGVE VAAMAGLPKE VVARARALLQ AMAARREGAL DAVLERLLAL
781 DPDRLTPLEA LRLLQELKAL ALGAPLDTMK G |
Storage Buffer |
20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 1 mM DTT, 50% Glycerol |
Reaction Buffer |
100mM KCl, 50 mM Tris-HCl, pH 8.5, 5~20 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 2% Glycerol, 65 °C |
Storage |
-20 to -80 °C. |
Shipping |
4°C or dry ice |
References |
- Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res. 2004 Nov 23;32(20):e162.
- Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Res. 2005 Mar 30;33(6):e55.
- MutS as a tool for mutation detection. Acta Biochim Pol. 2005;52(3):575-83. Epub 2005 Aug 4.
- One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA. Nucleic Acids Res. 2000 Apr 15;28(8):E36.
- Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology. Nature Methods - 4, 257 - 262 (2007) doi:10.1038/nmeth1007
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