| Service Name |
Cell-Free Protein Synthesis (CFPS) Service |
| Description |
Our comprehensive Cell-Free Protein Synthesis service provides rapid, flexible production of recombinant proteins without living cells. Utilizing advanced prokaryotic and eukaryotic lysate systems, we deliver high-quality proteins for research, structural biology, and drug discovery applications. With 15+ years of protein expression expertise, we specialize in challenging targets including membrane proteins, toxic proteins, and those requiring non-natural amino acid incorporation.
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| Key Advantages |
- Rapid Turnaround: Protein production in 1-2 weeks (vs 4-6 weeks for cell-based systems)
- High Success Rate: Natural protein folding with >90% active proteins
- Challenging Targets: Ideal for toxic, insoluble, or membrane proteins
- Custom Modifications: Incorporation of labeled amino acids, non-natural amino acids, or stable isotopes
- Scalable: From 100 μg to milligram quantities
- Multiple Systems: E. coli, wheat germ, mammalian, and insect lysates available
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| Workflow Timeline |
| Timeline |
Process |
| 1-2 weeks |
Template Preparation:
- PCR amplification (if using DNA template)
- Optional cloning into expression vector
- Sequence verification
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| 1 week |
Protein Production:
- Cell-free synthesis in selected system
- Scale-up optimization
- Purification via ÄKTA FPLC systems
- Quality control (SDS-PAGE, Western blot, activity assays)
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| Available Systems |
- Prokaryotic: NEB PURExpress, Qiagen EasyXpress, Roche ProteoMaster E. coli
- Eukaryotic: Roche RTS Wheat Germ, CellFree Sciences ENDEXT, Thermo Scientific Human In Vitro
- Specialty: Promega TNT, Invitrogen Expressway™
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| Applications |
- Structural biology (X-ray crystallography, NMR, cryo-EM)
- Antibody production and protein array development
- Drug discovery and high-throughput screening
- Enzyme engineering and characterization
- Incorporation of modified amino acids or stable isotopes
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| Deliverables |
- Purified protein in buffer of choice (0.1-10 mg typical yield)
- Complete QC package (SDS-PAGE, Western blot, activity data)
- ÄKTA FPLC chromatograms and purification report
- Expression optimization recommendations
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| Pricing |
| Service |
Price |
| Cloning/Subcloning |
$600 |
| Pilot Expression (100-500 μL) |
$1200 |
| Large-scale Production (1-5 mL) |
$1500-$2800 |
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| Ordering |
Submit protein sequence, accession number, or plasmid DNA to:
Protein Services, Excellgen, Inc.
15601 Crabbs Branch Way, Rockville, MD 20855-2743
For quotes: crm@excellgen.com
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