| Service Name |
Recombinant Protein and Antibody Expression Screening and Optimization |
| Description |
Our Recombinant Protein and Antibody Expression Screening and Optimization service is designed to maximize the yield, solubility, and functionality of your target proteins or antibodies. Using a systematic, high-throughput approach, we evaluate multiple expression systems, vectors, tags, and culture conditions to identify the optimal strategy for your specific project—minimizing development time and maximizing success for downstream scale-up and purification.
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| Key Features |
- Parallel screening of multiple expression hosts: E. coli, yeast, insect, and mammalian cells
- Evaluation of various vectors, promoters, fusion tags, and signal peptides
- Small-scale expression and solubility testing
- Optimization of induction, temperature, media, and culture conditions
- Rapid identification of the best expression construct and conditions
- Comprehensive data reporting for informed scale-up decisions
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| Workflow |
- Design and cloning of expression constructs
- Parallel transformation/transfection into selected host systems
- Small-scale expression and solubility screening
- Analysis by SDS-PAGE, Western blot, and activity assays (if applicable)
- Optimization of top candidates (expression conditions, tags, etc.)
- Recommendation of optimal construct and conditions for scale-up
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| Applications |
- Recombinant protein and antibody production for research, diagnostics, or therapeutics
- Structural biology, enzyme assays, and functional studies
- Antigen and antibody engineering
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| Deliverables |
- Comprehensive screening and optimization report
- Top-performing expression constructs and host strains/cell lines
- Small-scale expressed protein/antibody samples (if requested)
- Recommendations for large-scale expression and purification
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| Advantages |
- Accelerated project timelines with high-throughput screening
- Reduced risk of scale-up failure
- Expert guidance and technical support at every stage
- Cost-effective by minimizing wasted resources and maximizing yield
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Contact |
Contact us for a custom quote and to discuss your expression screening and optimization needs.
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