| Product Name |
EGFP Self-Amplifying mRNA |
| Description |
This self-amplifying mRNA encodes the enhanced green fluorescent protein (EGFP) along with four nonstructural proteins (nsP1–nsP4) derived from the positive-sense single-stranded RNA alphavirus Venezuelan equine encephalitis virus (VEEV). These nonstructural proteins mediate intracellular RNA replication, enabling prolonged and enhanced transgene expression.
|
| Modifications |
- 5' capped using the vaccinia capping enzyme system and 2'-O-methyltransferase to generate a Cap-1 structure
- 100% substitution with pseudouridine triphosphate (Ψ-UTP)
- 30% substitution with 5-methylcytidine triphosphate (5mCTP)
- Poly(A) tail: 180 nucleotides
|
| Application |
Reporter assay and gene expression studies |
| Concentration |
~1 µg/µL |
| Transfection Protocol |
Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
|
| QC |
Agarose gel electrophoresis |
| Shipping |
Dry ice |
| Storage |
-80 °C |