| Product Name |
Enhanced Green Fluorescent Protein (EGFP) mRNA |
| Description |
This in vitro transcribed mRNA encodes an enhanced variant of green fluorescent protein (EGFP), optimized for high-level expression and fluorescence intensity. EGFP mRNA serves as a robust reporter for evaluating transfection efficiency, tracking gene expression, and validating mRNA delivery methods in mammalian systems.
|
| Modifications |
- 5' capped with ARCA (Anti-Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'-OMeA)pG, Cap 1)
- Incorporation of modified nucleotides: Pseudo-UTP and 5-methyl-CTP (5mCTP)
- Poly(A) tail: 180 nucleotides
|
| Application |
Reporter assay for transfection and expression studies |
| Concentration |
~1 µg/µL |
| Transfection Protocol |
Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
|
| Quality Control |
Agarose gel electrophoresis |
| Shipping |
Dry ice |
| Storage |
-80 °C |