Klenow Fragment

Catalog #: EG-1055

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Description	Klenow Fragment is a truncated verson of E coli DNA Polymerase I. The enzyme exhibits 5'→3' polymerase (DNA synthesis) and proofreading (3′→5′) nuclease activities, but lacks 5'→3' exonuclease activity.
Applications	Removeal of 3´ overhangs to form blunt ends Random-primed DNA labeling Labeling by fill-in 5'-overhangs of dsDNA to form blunt ends DNA sequencing by the Sanger method Site-specific mutagenesis of DNA with synthetic oligonucleotides Second strand synthesis of cDNA
Source	A recombinant E. coli strain carrying the Klenow Fragment of E coli PolA gene.
Unit Definition	1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.
Components	Klenow Fragment: 5,000 U/ml in 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl₂, 10 mM DTT, pH 7.9 @ 25°C
Quality Control	The absence of endodeoxyribonucleases confirmed by appropriate quality test. Functionally tested for fill in of 5'-overhanging DNA termini and for random primed DNA labeling.
Storage Condition	-20 °C

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