| Product Name |
Klenow Fragment (3’ to 5’ exo-) |
| Catalog Number |
EG-1056 |
| Description |
Klenow Fragment (3’ to 5’ exo-), a recombinant N-terminal truncation of Escherichia coli DNA Polymerase I with mutations (D355A, E357A), retains 5’ to 3’ polymerase activity but lacks 5’ to 3’ exonuclease and 3’ to 5’ proofreading exonuclease activities, ideal for DNA labeling and sequencing applications. |
| Applications |
- Random-primed DNA labeling
- DNA sequencing by the Sanger method
- Second-strand cDNA synthesis
- Fill-in of 5’ overhangs of dsDNA to form blunt ends
- Strand displacement amplification (SDA)
|
| Source |
Recombinant Escherichia coli expressing the mutated Klenow Fragment of the polA gene |
| Unit Definition |
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard DNA polymerase assay conditions. |
| Components |
- Klenow Fragment (3’ to 5’ exo-): 5,000 units/mL in 25 mM Tris-HCl (pH 7.4 at 25°C), 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol
- 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl (pH 7.9 at 25°C), 100 mM MgCl2, 10 mM DTT
|
| Quality Control |
- Absence of endo- and exodeoxyribonucleases confirmed by appropriate quality tests
- Functionally validated for random-primed DNA labeling
|
| Storage |
-20°C |
| Shipping |
Dry ice |