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phi29 (φ29) DNA Polymerase

Catalog #: EG-1057

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Description	φ29 DNA Polymerase is a highly processive polymerase from Bacillus Subtilis phage φ29. It has exceptional strand displacement and processive synthesis properties, which allows for highly efficient isothermal DNA amplification. The enzyme possesses a 3'→5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA, allowing high-fidelity DNA synthesis.
Applications	Rolling circle amplification (RCA): generation of periodic DNA nanotemplates Multiple displacement amplification (MDA) Unbiased amplification of whole genome (WGA): amplification of DNA for SNP and STR detection cell-free amplification of DNA from single cells pathogenic organisms or metagenomes amplification of DNA from filter paper blood spot samples DNA template preparation for sequencing Protein-primed DNA amplification In situ genotyping with padlock probes Recombination based-cloning Cell-free cloning of lethal DNA RNA-primed DNA amplification
Source	A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
Components	phi29 DNA Polymerase: 100,000 U/ml in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween-20, 0.5% NP-40, 50% glycerol, pH 7.4 @ 25°C 10X Reaction Buffer: 500 mM Tris-HCl, 100 mM (NH₄)₂SO₄, 40 mM DTT, 100 mM MgCl₂, pH 7.5 @ 25°C
Quality Control	The absence of endodeoxyribonucleases confirmed by appropriate quality tests.
Storage Condition	-20 °C

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