| Product Name |
φ29 DNA Polymerase |
| Catalog Number |
EG-1057 |
| Description |
φ29 DNA Polymerase, a highly processive enzyme from Bacillus subtilis bacteriophage φ29 expressed recombinantly in Escherichia coli, catalyzes high-fidelity 5’ to 3’ DNA synthesis with 3’ to 5’ proofreading exonuclease activity on single-stranded DNA and exceptional strand displacement, ideal for isothermal DNA amplification. |
| Applications |
- Rolling circle amplification (RCA) for generating periodic DNA nanotemplates
- Multiple displacement amplification (MDA)
- Whole-genome amplification (WGA) for:
- SNP and STR detection
- Cell-free amplification from single cells
- Pathogenic organisms or metagenomes
- Amplification from filter paper blood spot samples
- DNA template preparation for sequencing
- Protein-primed DNA amplification
- In situ genotyping with padlock probes
- Recombination-based cloning
- Cell-free cloning of lethal DNA
- RNA-primed DNA amplification
|
| Source |
Recombinant Escherichia coli expressing the φ29 DNA polymerase gene from Bacillus subtilis bacteriophage φ29 |
| Unit Definition |
One unit is defined as the amount of enzyme that incorporates 0.5 pmol of dNTPs into acid-insoluble material in 10 minutes at 30°C under standard DNA polymerase assay conditions. |
| Components |
- φ29 DNA Polymerase: 100,000 units/mL in 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Tween 20, 0.5% (v/v) Nonidet P-40, 50% (v/v) glycerol
- 10X Reaction Buffer: 500 mM Tris-HCl (pH 7.5 at 25°C), 100 mM (NH4)2SO4, 100 mM MgCl2, 40 mM DTT
|
| Quality Control |
- Absence of endodeoxyribonucleases confirmed by appropriate quality tests
- Functionally validated for rolling circle amplification (RCA) and multiple displacement amplification (MDA)
|
| Storage |
-20°C |
| Shipping |
Dry ice |