| Product Name |
Pfu DNA Polymerase |
| Catalog Number |
EG-1028 |
| Description |
Pfu DNA polymerase, a thermostable enzyme with 3’ to 5’ proofreading activity, derived from the pol gene of Pyrococcus furiosus expressed in Escherichia coli, catalyzes high-fidelity DNA synthesis at an optimal temperature of ~75°C with a low error rate. |
| Applications |
- High-fidelity PCR and primer-extension reactions
- Generation of PCR products for cloning and expression
- Blunt-end PCR cloning
- RT-PCR for cDNA cloning and expression
- Site-directed mutagenesis
|
| Source |
Recombinant Pyrococcus furiosus pol gene expressed in Escherichia coli |
| Unit Definition |
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 74°C under standard DNA polymerase assay conditions. |
| Components |
- Pfu DNA Polymerase: 5 units/µL in 20 mM Tris-HCl (pH 8.2), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.1% Nonidet P-40, 0.1% Tween 20, 50% glycerol, and enzyme stabilizers
- 10X Reaction Buffer: 200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM (NH4)2SO4, 100 mM KCl, 1% Triton X-100, 1 mg/mL BSA, 20 mM MgSO4
|
| Storage |
-20°C |
| Shipping |
Dry ice |
|