| Product Name |
Taq DNA Polymerase |
| Catalog Number |
EG-1030 |
| Description |
Taq DNA Polymerase, a thermostable enzyme derived from the pol gene of Thermus aquaticus YT-1 expressed in Escherichia coli, catalyzes 5’ to 3’ DNA synthesis with no 3’ to 5’ proofreading activity, low 5’ to 3’ exonuclease activity, and deoxynucleotidyl transferase activity, resulting in 3’-dA overhangs on PCR products, with a molecular weight of 94 kDa. |
| Features |
- Thermostable with a half-life exceeding 40 minutes at 95°C
- Generates PCR products with 3’-dA overhangs
- Purity >99% by SDS-PAGE
- No detectable Escherichia coli 16S rDNA contamination by RT-PCR
|
| Applications |
- Routine PCR amplification of DNA fragments up to 5 kb
- TA cloning
|
| Source |
Recombinant Escherichia coli expressing the Thermus aquaticus YT-1 pol gene |
| Unit Definition |
One unit is defined as the amount of enzyme that incorporates 15 nmol of dNTPs into acid-insoluble material in 30 minutes at 75°C under standard DNA polymerase assay conditions. |
| Components |
- Taq DNA Polymerase: 5 units/µL in 20 mM Tris-HCl (pH 7.5), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% (v/v) Nonidet P-40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol, and stabilizers
- 10X Standard Taq Buffer: 100 mM Tris-HCl (pH 8.3 at 25°C), 500 mM KCl, 15 mM MgCl2
|
| Storage |
-20°C |
| Shipping |
Dry ice |
|