| Service Name |
Adaptation of CHO Cells to Suspension Culture |
| Description |
Our Adaptation of CHO Cells to Suspension Culture service transitions adherent Chinese Hamster Ovary (CHO) cells into robust, serum-free suspension cultures optimized for large-scale protein expression and bioprocessing. This service is ideal for clients seeking to scale up recombinant protein or antibody production using CHO cells in bioreactors or shake flasks. We use stepwise adaptation protocols and optimized media to ensure healthy, high-viability cell populations suitable for further genetic engineering or production runs.
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| Key Features |
- Stepwise adaptation of adherent CHO cells to serum-free suspension culture
- Selection of high-viability, fast-growing suspension cell lines
- Optimization of culture conditions and media formulation
- Compatible with a variety of CHO cell lines (e.g., CHO-K1, CHO-S, DG44)
- Ready for scale-up in shake flasks or bioreactors
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| Workflow |
- Initial assessment of parental adherent CHO cell line
- Stepwise reduction of serum and transition to suspension media
- Monitoring and selection of healthy, suspension-adapted cells
- Expansion and stabilization of suspension culture
- Quality control and viability assessment
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| Deliverables |
- Suspension-adapted CHO cell aliquots (cryopreserved)
- Adaptation report with culture conditions and recommendations
- Growth and viability data
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| Applications |
- Large-scale recombinant protein or antibody production
- Bioreactor and shake flask culture
- Cell line engineering and stable cell pool generation
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Contact |
Contact us for a custom quote and to discuss your specific CHO cell adaptation needs.
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