| Service Name |
5–10 L Suspension Culture & Purification Service |
| Description |
Our 5–10 L Suspension Culture & Purification Service is designed for large-scale recombinant protein production and purification from suspension cultures. Ideal for projects requiring milligram to multi-milligram quantities of protein, this service includes optimization and scale-up of expression in your preferred host (e.g., E. coli, yeast, insect, or mammalian cells), followed by purification using advanced ÄKTA FPLC (Fast Protein Liquid Chromatography) systems. The workflow is tailored to maximize protein yield, purity, and biological activity for downstream research, assay development, or preclinical studies.
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| Key Features |
- Large-scale suspension culture: 5–10 liters
- Customizable expression in E. coli, yeast, insect, or mammalian cells
- High-yield, high-purity protein production
- Purification via affinity, ion exchange, size exclusion, or multi-step FPLC workflows
- Compatible with a variety of affinity tags (His, GST, MBP, Strep, Fc, etc.)
- Optional tag removal and protein polishing steps
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| Workflow |
- Expression optimization and small-scale testing
- Large-scale (5–10 L) suspension culture expression
- Cell harvest and lysis
- FPLC purification (affinity, ion exchange, size exclusion, or combination)
- Buffer exchange and concentration (if required)
- Comprehensive quality control
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| Quality Control |
- SDS-PAGE and Coomassie staining (>95% purity)
- Western blot analysis (if antibody is available)
- Protein concentration determination
- Chromatograms and process documentation
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| Deliverables |
- Pooled, purified protein in your specified buffer and concentration
- Comprehensive quality control report (SDS-PAGE, purity, yield, activity, etc.)
- Chromatograms and method summary
- Optional: expression plasmid, cell pellets, or lysate upon request
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| Sample Requirements |
- Expression plasmid or gene sequence
- Preferred tag and purification method (if applicable)
- Any special buffer or storage requirements
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| Applications |
- Structural biology (X-ray, NMR, cryo-EM)
- Enzyme assays and functional studies
- Antibody production
- Preclinical research and drug screening
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Contact |
Contact us for a custom quote and to discuss your specific project requirements.
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