| Service Name |
IgE Purification from Culture Medium |
| Description |
Our IgE purification service isolates and purifies immunoglobulin E (IgE) antibodies from mammalian cell culture supernatant. Using optimized affinity chromatography and polishing steps, we deliver high-purity, functional IgE suitable for research, diagnostics, and functional assays.
|
| Workflow |
- Clarification of culture medium by centrifugation and filtration
- Affinity purification using anti-IgE or custom resin
- Polishing by size exclusion or ion exchange chromatography (AKTA FPLC)
- Buffer exchange and concentration
- Quality control (SDS-PAGE, Western blot, yield determination)
|
| Advantages |
- High specificity and purity (>95%)
- Preserves IgE structure and function
- Scalable for small or large volumes
- Fast turnaround and expert technical support
|
| Deliverables |
- Pooled, purified IgE antibody (>95% purity)
- Quality control data (SDS-PAGE, Western blot, yield)
- Chromatograms and process documentation
|
| Required Materials |
- Cell culture supernatant containing secreted IgE
- Information on cell line and medium composition
- Desired buffer and concentration (if specific requirements)
|
| Applications |
- Allergy and immunology research
- Diagnostic assay development
- Functional and binding studies
|
| Consultation & Support |
Free project consultation and technical support throughout your project.
|
| Quotation & Ordering |
- Request a quotation form: crm@excellgen.com
- Submit the completed form and PO/credit card info by email, fax, or phone
- Ship samples with a hard copy of the completed form to:
Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743
|