| Service Name |
MembraneExpressTM Functional Membrane Protein Expression and Production |
| Description |
Our functional membrane protein production system is based on state-of-the-art virus-like particle (VLP) technology developed by Excellgen scientists. This platform enables the expression of functional membrane proteins (e.g., GPCRs, receptor kinases, ion channels, viral envelopes) in their native context for downstream assays and screening.
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| Workflow & Timeline |
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Cloning and Subcloning (2 weeks):
- PCR amplification using high-fidelity DNA polymerase
- Cloning PCR product into an expression vector
- Restriction digestion analysis and sequencing to confirm correct sequence and frame
- Preparation of plasmid DNA
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Expression Optimization (2 weeks):
- Transfection of CHO or HEK293 cells with plasmid DNA
- Assessment of protein expression level by SDS-PAGE, dot-blot, or Western blot analysis
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Large Scale Expression and Purification (2 weeks):
- Transfect 500 ml CHO or HEK293 cells with plasmid DNA (or use baculovirus-Sf9 system as needed)
- Culture cells using serum-free medium
- Purify protein using AKTA FPLC systems
- Dialysis and concentration
- SDS-PAGE or Western blot analysis of the purified protein
- Determination of protein concentration
- Delivery of purified, ready-to-use protein
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| Advantages |
- Rapid delivery: VLP pseudotyped with membrane proteins can be produced in as little as 2 weeks.
- Scalable production in HEK293 cells or baculovirus-Sf9 system.
- Suitable for functional membrane proteins in their native context.
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| Deliverables |
- Functional virus-like particles containing the membrane protein of interest
- QC data (SDS-PAGE, Western blot, concentration)
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| Required Materials |
- Plasmid containing gene of interest plus complete vector sequence (electronic format)
- or protein sequence for gene synthesis (additional fee applies)
- or accession number
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| Quotation & Ordering |
- Request a quotation form from customer service: crm@excellgen.com
- Submit the completed form and PO/credit card info by email, fax, or phone
- Ship samples with a hard copy of the completed form to:
Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743
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| Base Price |
- Cloning: $600
- Pilot Expression and Analysis: $1200
- Development of Optimal Expression & Purification Protocol: $2800
- Large Scale Expression and Purification: $1000 for 500 ml, $1500 for 1 L
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