| Service Name |
Protein Expression and Purification using Bacterial Expression System |
| Description |
Our Bacterial Expression and Purification Service offers rapid, cost-effective production of recombinant proteins in E. coli using advanced fusion tags and dual-tag vectors for enhanced expression, solubility, and streamlined purification. This platform is ideal for research, assay development, and preparative-scale protein production, delivering high-purity proteins suitable for downstream analysis or functional studies.
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| Key Features & Advantages |
- Enhanced expression with GST, MBP, and NusA fusion tags
- Dual-tag vectors (His-GST, His-MBP, His-Strep) for simple and efficient purification
- Optional fluorescent (GFP, RFP), luciferase, or detection tags (HA, Myc, Flag) for downstream assays
- High-yield, scalable production in E. coli
- AKTA FPLC purification for >95% purity
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| Workflow & Timeline |
- Cloning (2 weeks):
- Gene cloning or subcloning into expression vector
- Verification by restriction digestion and sequencing
- Plasmid DNA preparation
- Expression Optimization (1–2 weeks):
- Transformation of competent cells
- Optimization of induction (time, temperature, IPTG concentration)
- Expression analysis by SDS-PAGE, dot-blot, or Western blot
- Large-Scale Expression & Purification (1–2 weeks):
- Large-scale culture and protein expression
- Purification using AKTA FPLC
- Dialysis, concentration, and QC (SDS-PAGE, Western blot, protein quantitation)
- Delivery of purified, ready-to-use protein
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| Deliverables |
- >3 mg purified recombinant protein (>95% purity)
- Quality control data (SDS-PAGE, Western blot, yield)
- Chromatograms and process documentation
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| Required Materials |
- 5 µg plasmid containing gene of interest (with complete vector sequence in electronic format)
- or protein sequence for gene synthesis (additional fee applies)
- or accession number for cDNA sourcing
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| Applications |
- Research and assay development
- Structural biology and functional studies
- Antibody production and immunization
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Quotation & Ordering |
- Request a quotation form from customer service: crm@excellgen.com
- Submit the completed form and PO/credit card info by email, fax, or phone
- Ship samples with a hard copy of the completed form to:
Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743
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