| Service Name |
Custom Protein Purification Services using ÄKTA FPLC Systems |
| Description |
Our Custom Protein Purification Service utilizes advanced ÄKTA FPLC (Fast Protein Liquid Chromatography) systems for high-yield, high-purity protein isolation from a wide range of biological sources. We offer purification strategies including affinity, ion-exchange, hydrophobic interaction, and size exclusion chromatography, tailored to your protein and application needs. Our experienced team supports both standard and complex workflows, including tag removal and antibody purification.
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| Supported Tags & Affinity Purification |
- Fc
- 6X His
- GST
- MBP
- Strep(II)
- Protein A
- Biotin
- E-tag
- Calmodulin-binding peptide
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| Antibody Purification |
- Monoclonal and polyclonal antibody purification using protein A/G columns
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| Additional Services |
- Tag or fusion protein removal by protease digestion (if protease site is available), followed by further purification
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| Quality Control |
- Purity analysis by SDS-PAGE and Coomassie staining (>95% purity)
- Western blot analysis (if antibody is available)
- Protein concentration determination
- Chromatograms provided with each project
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| Supported Sample Types |
- Tissue culture supernatant
- Cell pellet
- Cell extracts or homogenates
- Tissue extracts or homogenates
- Biological fluids (serum, plasma, urine, CSF, saliva, BALF)
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| Required Materials |
- Sample(s) to be purified
- Protein information (sequence, tag, buffer, salt concentration, expression level, pI, solubility, etc.)
- Antibody (if a common purification tag is not used)
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| Deliverables |
- Purified protein (>95% purity, custom buffer and concentration)
- QC data (SDS-PAGE, Western blot, protein concentration)
- Chromatograms
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| Consultation & Ordering |
- Free project consultation and technical support
- To request a quote, please contact us for a quotation form
- Order by submitting the completed quotation form and purchase order or credit card information
- Ship samples with a hard copy of the completed quotation form to our Protein Services team
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| Contact |
Contact us for a custom quote and to discuss your purification requirements.
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