| Service Name |
Protein Expression and Purification Service |
| Description |
Our Protein Expression and Purification Service provides comprehensive, customizable solutions for the production of high-quality recombinant proteins tailored to your research needs. We offer a range of expression systems—including E. coli, yeast, insect, and mammalian cells—and purification strategies to deliver proteins with high purity, yield, and activity. From gene synthesis and vector construction to scale-up and quality control, our experienced team ensures reliable, reproducible results for applications in structural biology, functional assays, drug discovery, and more.
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| Key Features |
- Multiple expression systems: E. coli, yeast, baculovirus-insect cells, mammalian cells
- Customizable tags and fusion partners (e.g., His, GST, MBP, FLAG)
- Flexible scale: from milligram to multi-gram quantities
- Affinity, ion exchange, size exclusion, and custom purification methods
- Endotoxin removal and tag cleavage options available
- Comprehensive quality control: SDS-PAGE, Western blot, activity assays, mass spectrometry
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| Workflow |
- Gene synthesis or subcloning
- Vector construction and sequence verification
- Small-scale expression testing and optimization
- Large-scale protein expression
- Protein purification and polishing
- Quality control and final reporting
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| Applications |
- Structural biology (X-ray, NMR, cryo-EM)
- Enzymatic and functional assays
- Antibody production
- Drug screening and target validation
- Diagnostics and industrial applications
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| Deliverables |
- Purified recombinant protein (custom buffer and concentration)
- Quality control report (SDS-PAGE, purity, yield, activity, etc.)
- Optional: expression plasmid, cell pellets, or lysate upon request
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| Consultation & Support |
Free project consultation and technical support throughout the process.
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| Contact |
Contact us for a custom quote and to discuss your specific project requirements.
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