| Product Name |
Firefly Luciferase mRNA, Cy5-Modified |
| Overview |
This product consists of in vitro transcribed firefly luciferase messenger RNA (mRNA) incorporating Cy5-labeled nucleotides for direct fluorescence detection. The Cy5 modification enables visualization of mRNA uptake and intracellular distribution using fluorescence microscopy with a Cy5 filter set. Quantitative assessment of translation efficiency can be performed via a luciferase reporter assay.
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| Key Features |
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5' Cap Structure: Available with either ARCA (Anti-Reverse Cap Analog; m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'-O-methyl-A)pG, Cap 1) for enhanced stability and translational efficiency.
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Nucleotide Modifications: Incorporates Cy5-UTP and N1-methyl-pseudouridine-5'-triphosphate at a 1:3 molar ratio to improve mRNA stability and reduce innate immune activation.
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Poly(A) Tail: 180 nucleotides in length for optimal translational performance.
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| Applications |
- Evaluation of mRNA delivery efficiency via fluorescence microscopy
- Quantitative luciferase reporter assays for transfection and expression analysis
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| Concentration |
~1 µg/µL |
| Recommended Transfection Protocol |
Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
|
| Quality Control |
Assessed by agarose gel electrophoresis |
| Shipping Conditions |
Shipped on dry ice |
| Storage |
Store at –80 °C |